產(chǎn)品介紹

BD 人iPSC分選和分析試劑盒562626

BD Stemflow™ Human Induced Pluripotent Stem Cell Analysis and Sorting Kit

BD Stemflow™ 人誘導(dǎo)多能干細(xì)胞分選與分析試劑盒

產(chǎn)品概述

本試劑盒包含 小鼠單克隆抗體偶聯(lián)物組合，用于 人誘導(dǎo)多能干細(xì)胞（hiPSC）培養(yǎng)物 及 重編程過(guò)程中的細(xì)胞 的分選與分析。

主要應(yīng)用

• hiPSC 分選：從異質(zhì)性細(xì)胞群中分選 hiPSC

• 重編程過(guò)程監(jiān)測(cè)：鑒定處于重編程不同階段的細(xì)胞

• 表面標(biāo)志物分析：基于 3 種關(guān)鍵表面標(biāo)志物 進(jìn)行流式檢測(cè)

試劑盒組成

1. 抗體偶聯(lián)物（靶向 hiPSC 特異性表面標(biāo)志物）

2. 同型對(duì)照（Isotype Controls）

3. BD™ CompBead Plus（補(bǔ)償微球，用于流式補(bǔ)償調(diào)節(jié)）

實(shí)驗(yàn)流程

1. 細(xì)胞準(zhǔn)備：按標(biāo)準(zhǔn)重編程方案獲得 hiPSC 或重編程中細(xì)胞

2. 抗體標(biāo)記：使用試劑盒內(nèi)抗體偶聯(lián)物進(jìn)行染色

3. 流式分選/分析：通過(guò) FACS 或流式細(xì)胞術(shù) 鑒定目標(biāo)細(xì)胞群

優(yōu)勢(shì)特點(diǎn)

? 高特異性：優(yōu)化的抗體組合確保 hiPSC 精準(zhǔn)分選
? 標(biāo)準(zhǔn)化操作：包含對(duì)照及補(bǔ)償微球，減少實(shí)驗(yàn)誤差
? 兼容性強(qiáng)：適用于 hiPSC 培養(yǎng)、重編程效率評(píng)估 等研究

BD 人iPSC分選和分析試劑盒562626

The BD Stemflow™ Human iPSC Sorting and Analysis Kit contains a combination of mouse monoclonal antibody conjugates for the sorting and analysis of induced human pluripotent stem cell (hiPSC) cultures and cells in the process of being reprogrammed.  Following established reprogramming protocols, hiPSCs are sorted or analyzed from a heterogeneous cell culture utilizing the included antibody conjugates to three cell surface markers.  The antibody specificities that are provided in this kit and the cell populations they can identify are listed in the table below.  The kit also includes Isotype Controls and BD™ CompBead Plus.