產(chǎn)品介紹

BD Stemflow™ 人神經(jīng)細(xì)胞分選試劑盒562271

BD Stemflow™ Human Neural Cell Sorting Kit

描述

產(chǎn)品用途

本試劑盒專為以下應(yīng)用設(shè)計(jì)：

• 神經(jīng)干細(xì)胞（NSCs）分選：從人多能干細(xì)胞（PSCs）誘導(dǎo)來(lái)源的異質(zhì)性細(xì)胞群中分選NSCs

• 神經(jīng)元與膠質(zhì)細(xì)胞分選：從已分化的NSCs中分離神經(jīng)元和膠質(zhì)細(xì)胞

技術(shù)優(yōu)勢(shì)

• 標(biāo)準(zhǔn)化分選：通過(guò)5種細(xì)胞表面標(biāo)志物的抗體偶聯(lián)物進(jìn)行分選，顯著降低傳統(tǒng)方法（如人工分離）導(dǎo)致的批次間差異

• 多功能應(yīng)用：其中4種抗體偶聯(lián)物還可用于從分化的NSCs中區(qū)分神經(jīng)元和膠質(zhì)細(xì)胞

工作原理

1. 神經(jīng)誘導(dǎo)：用戶需先通過(guò)成熟的神經(jīng)誘導(dǎo)方案獲得含NSCs的異質(zhì)性細(xì)胞群

2. 抗體標(biāo)記：利用試劑盒提供的特異性抗體偶聯(lián)物標(biāo)記目標(biāo)細(xì)胞

3. 流式分選：基于表面標(biāo)志物表達(dá)譜實(shí)現(xiàn)高純度NSCs、神經(jīng)元或膠質(zhì)細(xì)胞的分離

兼容性說(shuō)明

• 適用于人多能干細(xì)胞（hESCs/iPSCs）來(lái)源的NSCs分選

• 支持后續(xù)神經(jīng)元/膠質(zhì)細(xì)胞亞群分析

The BD Stemflow™ Neural Cell Isolation Kit was designed to allow the isolation of neural stem cells (NSCs) derived from human pluripotent stem cells or the isolation of neurons and glial cells from differentiated NSCs.  Following a variety of established neural induction protocols, NSCs are sorted from the heterogeneous cell culture utilizing the included antibody conjugates to five cell surface markers.  This method greatly reduces the amount of variability between NSC isolations as opposed to traditional methods like manual isolation.  In addition, four of the five included conjugates can be used to isolate neurons from glia from differentiated NSCs.

BD Stemflow™ 人神經(jīng)細(xì)胞分選試劑盒562271

BD Stemflow™ Human Neural Cell Sorting Kit

準(zhǔn)備和存儲(chǔ)

在 4°C 下原液保存，避免長(zhǎng)時(shí)間光照。不要凍結(jié)。